Coding

Part:BBa_K2952009:Design

Designed by: Charlotte von Koppenfels   Group: iGEM19_Edinburgh_UG   (2019-10-04)


NiFe Hydrogenase SHI subunit 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 60
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

RFC10 and TypeIIS compatible. Codon usage improved for expression in Rhodobacter sphaeroides.


Source

genomic sequence, Pyroccocus furiosus

References